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doi: 10.1016/j.neulet.2013.04.054

GST by itself will have pretty high binding capacity, whereas the larger the protein of interest that is fused to GST is, the lower the binding capacity will be

KCl 2.7 mM, KH 2 PO 4 1.5 mM, NaCl 136 mM, Na 2 HPO 4 2 H 2 O 6.5 mM, pH 7.4) containing 3% bovine serum albumin (BSA-PBS) for 1 h at room temperature and were then incubated with a rabbit anti-claudin 5 primary antibody diluted in 3% BSA-PBS (1:300) overnight at 4 C