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Unlike most of the growth factors that are ready to function upon secretion, TGF- is unique in that it is secreted as part of a latent complex that is stored in the extracellular matrix (ECM)

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It is important to remember that lipoproteins and other lipid material can rapidly clog chromatography columns, so it may be necessary to remove them before purification using precipitation agents like dextran sulfate or polyvinylpyrrolidone

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